Rabbit anti-Collagen 5 alpha 2 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Collagen 5 alpha 2
CAS Number
9007-83-4
Synonyms
Collagen alpha-2 (V) chain
Host
Rabbit
Reactivity
H, R, P
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Collagen 5 alpha 2. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IH, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
WB Description
Western blot analysis of Collagen 5 alpha 2 expression in H9C2 (A), PC3 (B), MCF7 (C) whole cell lysates.
IHC description
Immunohistochemical analysis of Collagen 5 alpha 2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description
Immunofluorescent analysis of Collagen 5 alpha 2 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
1290
SwissProt (Human)
P05997
Available Sizes
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
BNC400352-500 1x 500 µL
CD31 / PECAM-1 (158-2B3), CF640R conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC471003-100 1x 100 µL
Mucin 5AC (MUC5AC) (58M1), CF647 conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC740460-500 1x 500 µL
CD44 Standard (156-3C11), CF740 conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC680352-500 1x 500 µL
CD31 / PECAM-1 (158-2B3), CF568 conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC471081-500 1x 500 µL
CD45RO (190-2F2.5), CF647 conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC740017-100 1x 100 µL
Ep-CAM / CD326 (VU-1D9), CF740 conjugate, 0.1mg/mL
Sign In for Pricing
View Details