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Rabbit anti-DARPP32 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to DARPP32

CAS Number

9007-83-4

Synonyms

DARPP32; Protein phosphatase 1 regulatory subunit 1B; DARPP-32; Dopamine- and cAMP-regulated neuronal phosphoprotein

Host

Rabbit

Reactivity

H, M, R, P

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human DARPP32. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IH

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

WB Description

Western blot analysis of DARPP32 expression in mouse heart (A), rat heart (B) whole cell lysates.

IHC description

Immunohistochemical analysis of DARPP32 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

84152

Gene ID (Mouse)

19049

Gene ID (Rat)

360616

SwissProt (Human)

Q9UD71

SwissProt (Mouse)

Q60829

SwissProt (Rat)

Q6J4I0

Available Sizes

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