Human CD8 alpha&beta (CD8A&CD8B) Heterodimer Protein, His Tag&Tag Free (MALS verified)

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. CD8A forms disulfide-linked heterodimers with CD8B at the cell surface. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells. Additionally, CD8B plays a critical role in thymic selection of CD8+ T-cells. A palmitoylation site in the cytoplasmic tail of CD8B chain contributes to partitioning of CD8 into the plasma membrane lipid rafts where signaling proteins are enriched.

Product Specifications

Background

Specifications

The protein has a calculated MW of 19.5 kDa & 22.1 kDa. The protein migrates as 28-30 kDa and 31-33 kDa under reducing (R) condition, and 30-32 kDa and 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

Accession Number

P01732 & P10966-1

Expression Region

Ser 22 - Asp 182 & Leu 22 - Pro 170

Host

HEK293

Target

CD8 alpha & beta

Conjugation

Unconjugated

Tag

C-10xHis | Native

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Human CD8A&CD8B Heterodimer Protein, His Tag&Tag Free (Cat. No. CDA-H52W5) at 2 μg/mL (100 μL/well) can bind Anti-CD8 alpha Antibody, Mouse IgG2a (clone: OKT8) with a linear range of 0.06-4 ng/mL (QC tested).