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Rabbit anti-Histone H2B (AcK20) Antibody

Product Specifications

Background

Rabbit polyclonal antibody to Histone H2B (AcK20)

CAS Number

9007-83-4

Synonyms

HIST1H2BA; TSH2B; Histone H2B type 1-A; Histone H2B testis; Testis-specific histone H2B; HIST1H2BB; H2BFF; Histone H2B type 1-B; Histone H2B.1; Histone H2B.f; H2B/f; HIST1H2BC; H2BFL; HIST1H2BE; H2BFH; HIST1H2BF; H2BFG; HIST1H2BG; H2BFA; HIST1H2BI; H2BFK; Histone H2B type 1-C/E/F/G/I; Histone H2B.1 A; Histone H2B.a; H2B/a; Histone H2B.g; H2B/g; Histone H2B.h; H2B/h; Histone H2B.k; H2B/k; Histone H2B.l; H2B/l

Host

Rabbit

Reactivity

H, M, R

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Histone H2B with a site at AcK20. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IH

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

WB Description

Western blot analysis of Histone H2B (AcK20) expression in Hela (A), mouse lung (B), rat kidney (C), rat lung (D) whole cell lysates.

IHC description

Immunohistochemical analysis of Histone H2B (AcK20) staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

255626; 3018; 3017; 8339; 8343; 8344; 8346; 8347

Gene ID (Mouse)

319177; 319178; 319179

Gene ID (Rat)

24829

SwissProt (Human)

Q96A08; P33778; P62807

SwissProt (Mouse)

P70696; Q64475; Q6ZWY9

SwissProt (Rat)

Q00729

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