Rabbit anti-Hairless Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Hairless
CAS Number
9007-83-4
Synonyms
Protein hairless
Host
Rabbit
Reactivity
H, R
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Hairless. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IH, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/100 - 1/500)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
WB Description
Western blot analysis of Hairless expression in rat colon (A), A375 (B), MCF7 (C) whole cell lysates.
IHC description
Immunohistochemical analysis of Hairless staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description
Immunofluorescent analysis of Hairless staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
55806
SwissProt (Human)
O43593
SwissProt (Rat)
P97609
Available Sizes
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
E-UNEL-H0076-01 5x 96 Tests
Uncoated Human TARC (Thymus Activation Regulated Chemokine) ELISA Kit
Sign In for Pricing
View Details E-UNEL-H0076-02 15x 96 Tests
Uncoated Human TARC (Thymus Activation Regulated Chemokine) ELISA Kit
Sign In for Pricing
View Details 
BNC741492-100 1x 100 µL
PAX8 (Renal Cell Marker) (PAX8/1492), CF740 conjugate, 0.1mg/mL
Sign In for Pricing
View Details