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Rabbit anti-NMBR Antibody

Product Specifications

Background

Rabbit polyclonal antibody to NMBR

CAS Number

9007-83-4

Synonyms

Neuromedin-B receptor; NMB-R; Epididymis tissue protein Li 185a; Neuromedin-B-preferring bombesin receptor

Host

Rabbit

Reactivity

H, M, R

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NMBR. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

WB Description

Western blot analysis of NMBR expression in EC9706 (A), Hela (B), PC3 (C) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of NMBR staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

4829

Gene ID (Mouse)

18101

Gene ID (Rat)

25264

SwissProt (Human)

P28336

SwissProt (Mouse)

O54799

SwissProt (Rat)

P24053

Available Sizes

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