Rabbit anti-Frizzled 3 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Frizzled 3
CAS Number
9007-83-4
Synonyms
Frizzled-3; Fz-3; hFz3
Host
Rabbit
Reactivity
H, M
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Frizzled 3. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
WB Description
Western blot analysis of Frizzled 3 expression in U87MG (A), Hela (B), mouse brain (C) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of Frizzled 3 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
7976
Gene ID (Mouse)
14365
SwissProt (Human)
Q9NPG1
SwissProt (Mouse)
Q61086
Available Sizes
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