Rabbit anti-CHRM4 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to CHRM4
CAS Number
9007-83-4
Synonyms
Muscarinic acetylcholine receptor M4
Host
Rabbit
Reactivity
H, M, R
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CHRM4. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
WB Description
Western blot analysis of CHRM4 expression in C6 (A), SGC7901 (B), U87MG (C), A375 (D) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of CHRM4 staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
1132
Gene ID (Mouse)
12672
Gene ID (Rat)
25111
SwissProt (Human)
P08173
SwissProt (Mouse)
P32211
SwissProt (Rat)
P08485
Available Sizes
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