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Rabbit anti-GUF1 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to GUF1

CAS Number

9007-83-4

Synonyms

Translation factor GUF1 mitochondrial; Elongation factor 4 homolog; EF-4; GTPase GUF1; Ribosomal back-translocase

Host

Rabbit

Reactivity

H

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GUF1. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IH

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

WB Description

Western blot analysis of GUF1 expression in SHSY5Y (A) whole cell lysates.

IHC description

Immunohistochemical analysis of GUF1 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

60558

SwissProt (Human)

Q8N442

Available Sizes

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