Rabbit anti-DCR3 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to DCR3

CAS Number

9007-83-4

Synonyms

DCR3; TR6; Tumor necrosis factor receptor superfamily member 6B; Decoy receptor 3; DcR3; Decoy receptor for Fas ligand; M68

Host

Rabbit

Reactivity

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human DCR3. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IH, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/100 - 1/500)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

WB Description

Western blot analysis of DCR3 expression in HCT116 (A) whole cell lysates.

IHC description

Immunohistochemical analysis of DCR3 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description

Immunofluorescent analysis of DCR3 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.