Rabbit anti-DAB1 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to DAB1
CAS Number
9007-83-4
Synonyms
Disabled homolog 1
Host
Rabbit
Reactivity
H, M, R, Mk
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human DAB1. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
WB Description
Western blot analysis of DAB1 expression in U87MG (A), HCT116 (B) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of DAB1 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
1600
Gene ID (Mouse)
13131
Gene ID (Rat)
266729
SwissProt (Human)
O75553
SwissProt (Mouse)
P97318
SwissProt (Rat)
Q8CJH2
Available Sizes
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