What is the catalog number for Rabbit anti-CD25 Antibody?

The catalog number for Rabbit anti-CD25 Antibody is DL98820A.

What sizes are available for Rabbit anti-CD25 Antibody?

Rabbit anti-CD25 Antibody is available in the following size(s): 50 µL, 100 µL.

How many pack sizes does Rabbit anti-CD25 Antibody come in?

Rabbit anti-CD25 Antibody is available in 2 different pack sizes.

Is Rabbit anti-CD25 Antibody in stock?

Rabbit anti-CD25 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Rabbit anti-CD25 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to CD25

CAS Number

9007-83-4

Synonyms

Interleukin-2 receptor subunit alpha; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA; TAC antigen; p55; CD25

Host

Rabbit

Reactivity

H, R

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CD25. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

WB Description

Western blot analysis of CD25 expression in PMVEC (A), Jurkat (B) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of CD25 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.