Rabbit anti-NF-kappaB p100 (pS869) Antibody
Product Specifications
Background
Rabbit polyclonal antibody to NF-kappaB p100 (pS869)
CAS Number
9007-83-4
Synonyms
LYT10; Nuclear factor NF-kappa-B p100 subunit; DNA-binding factor KBF2; H2TF1; Lymphocyte translocation chromosome 10 protein; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2; Oncogene Lyt-10; Lyt10
Host
Rabbit
Reactivity
H, M
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human NF-kappaB p100 (pS869) . The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IH, IP
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/50 - 1/200), IP (1/10 - 1/100)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
WB Description
Western blot analysis of NF-kappaB p100 (pS869) expression in Myla2059 (A) whole cell lysates.
IHC description
Immunohistochemical analysis of NF-kappaB p100 (pS869) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
4791
Gene ID (Mouse)
18034
SwissProt (Human)
Q00653
SwissProt (Mouse)
Q9WTK5
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