Rabbit anti-Leptin Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Leptin
CAS Number
9007-83-4
Synonyms
OB; OBS; Leptin; Obese protein; Obesity factor
Host
Rabbit
Reactivity
H, M
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Leptin. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IH
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
WB Description
Western blot analysis of Leptin expression in LO2 (A), HCT116 (B), MCF7 (C), A549 (D) whole cell lysates.
IHC description
Immunohistochemical analysis of Leptin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
3952
Gene ID (Mouse)
16846
SwissProt (Human)
P41159
SwissProt (Mouse)
P41160
Available Sizes
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