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Rabbit anti-SPTBN5 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to SPTBN5

CAS Number

9007-83-4

Synonyms

BSPECV; HUBSPECV; HUSPECV; Spectrin beta chain non-erythrocytic 5; Beta-V spectrin

Host

Rabbit

Reactivity

H

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SPTBN5. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IH, IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/50 - 1/200), IC (1/100 - 1/500)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

WB Description

Western blot analysis of SPTBN5 expression in HEK293T (A), SGC7901 (B), Panc1 (C) whole cell lysates.

IHC description

Immunohistochemical analysis of SPTBN5 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description

Immunocytochemistry analysis of SPTBN5 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a HRP-conjugated secondary antibody in PBS at room temperature. DAB was used as the chromogen.

Gene ID (Human)

51332

SwissProt (Human)

Q9NRC6

Available Sizes

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