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Rabbit anti-APOBEC3D Antibody

Product Specifications

Background

Rabbit polyclonal antibody to APOBEC3D

CAS Number

9007-83-4

Synonyms

APOBEC3D; Probable DNA dC->dU-editing enzyme APOBEC-3D; APOBEC3F; DNA dC->dU-editing enzyme APOBEC-3F; Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3F

Host

Rabbit

Reactivity

H, M, R

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human APOBEC3D. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IH

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

WB Description

Western blot analysis of APOBEC3D expression in H1792 (A), SKOVCAR3 (B), H9C2 (C), MEF (D) whole cell lysates.

IHC description

Immunohistochemical analysis of APOBEC3D staining in human placenta formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

200316; 140564

SwissProt (Human)

Q96AK3; Q8IUX4

Available Sizes

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