Rabbit anti-RAD23A Antibody

Product Specifications

Background

Rabbit polyclonal antibody to RAD23A

CAS Number

9007-83-4

Synonyms

UV excision repair protein RAD23 homolog A; HR23A; hHR23A

Host

Rabbit

Reactivity

H, R, B, Mk

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human RAD23A. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

WB Description

Western blot analysis of RAD23A expression in Hela (A), HEK293T (B), rat testis (C) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of RAD23A staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.