What is the catalog number for Rabbit anti-ECRG4 Antibody?

The catalog number for Rabbit anti-ECRG4 Antibody is DL98663A.

What sizes are available for Rabbit anti-ECRG4 Antibody?

Rabbit anti-ECRG4 Antibody is available in the following size(s): 50 µL, 100 µL.

How many pack sizes does Rabbit anti-ECRG4 Antibody come in?

Rabbit anti-ECRG4 Antibody is available in 2 different pack sizes.

Is Rabbit anti-ECRG4 Antibody in stock?

Rabbit anti-ECRG4 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Rabbit anti-ECRG4 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to ECRG4

CAS Number

9007-83-4

Synonyms

ECRG4; Augurin; Esophageal cancer-related gene 4 protein

Host

Rabbit

Reactivity

H, M, R

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ECRG4. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IH, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/100 - 1/500)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

WB Description

Western blot analysis of ECRG4 expression in U87MG (A), HEK293T (B), A549 (C), PC12 (D), CT26 (E) whole cell lysates.

IHC description

Immunohistochemical analysis of ECRG4 staining in human testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description

Immunofluorescent analysis of ECRG4 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.