Rabbit anti-Focal Adhesion Kinase Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Focal Adhesion Kinase
CAS Number
9007-83-4
Synonyms
FAK; FAK1; Focal adhesion kinase 1; FADK 1; Focal adhesion kinase-related nonkinase; FRNK; Protein phosphatase 1 regulatory subunit 71; PPP1R71; Protein-tyrosine kinase 2; p125FAK; pp125FAK
Host
Rabbit
Reactivity
H, M, R
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Focal Adhesion Kinase. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IH
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
WB Description
Western blot analysis of Focal Adhesion Kinase expression in SKOVCAR3 (A), LOVO (B), Beas2B (C), A549 (D), PC3 (E), NIH3T3 (F), CT26 (G) whole cell lysates.
IHC description
Immunohistochemical analysis of Focal Adhesion Kinase staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
5747
Gene ID (Mouse)
14083
Gene ID (Rat)
25614
SwissProt (Human)
Q05397
SwissProt (Mouse)
P34152
SwissProt (Rat)
O35346
Available Sizes
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