Rabbit anti-IP3R Antibody

Product Specifications

Background

Rabbit polyclonal antibody to IP3R

CAS Number

9007-83-4

Synonyms

INSP3R1; Inositol 145-trisphosphate receptor type 1; IP3 receptor isoform 1; IP3R 1; InsP3R1; Type 1 inositol 145-trisphosphate receptor; Type 1 InsP3 receptor

Host

Rabbit

Reactivity

H, M, R

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human IP3R. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IH, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/100 - 1/500)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

WB Description

Western blot analysis of IP3R expression in mouse brain (A), rat brain (B), U87MG (C), SGC7901 (D) whole cell lysates.

IHC description

Immunohistochemical analysis of IP3R staining in human cervix cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description

Immunofluorescent analysis of IP3R staining in COS7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.