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Rabbit anti-PAK4/5 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to PAK4/5

CAS Number

9007-83-4

Synonyms

KIAA1142; Serine/threonine-protein kinase PAK 4; p21-activated kinase 4; PAK-4

Host

Rabbit

Reactivity

H, M, R, B

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PAK4/5. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IC (1/100 - 1/500)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

WB Description

Western blot analysis of PAK4/5 expression in C6 (A), CT26 (B), A2780 (C), HCT116 (D), PC3 (E) whole cell lysates.

IF/ICC description

Immunocytochemistry analysis of PAK4/5 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a HRP-conjugated secondary antibody in PBS at room temperature. DAB was used as the chromogen.

Gene ID (Human)

10298

Gene ID (Mouse)

70584

SwissProt (Human)

O96013; Q9P286

SwissProt (Mouse)

Q8BTW9

Available Sizes

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