Rabbit anti-PKC mu (pS205) Antibody

Product Specifications

Background

Rabbit polyclonal antibody to PKC mu (pS205)

CAS Number

9007-83-4

Synonyms

PKD; PKD1; PRKCM; Serine/threonine-protein kinase D1; Protein kinase C mu type; Protein kinase D; nPKC-D1; nPKC-mu

Host

Rabbit

Reactivity

H, M, R

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PKC mu (pS205) . The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IH

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

WB Description

Western blot analysis of PKC mu (pS205) expression in HEK293T-PMA 10min (A), HEK293T-PMA 30min (B), H9C2 (C), NIH3T3 (D) whole cell lysates.

IHC description

Immunohistochemical analysis of PKC mu (pS205) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.