Rabbit anti-Progesterone Receptor (pS400) Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Progesterone Receptor (pS400)
CAS Number
9007-83-4
Synonyms
NR3C3; Progesterone receptor; PR; Nuclear receptor subfamily 3 group C member 3
Host
Rabbit
Reactivity
H, M, R
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Progesterone Receptor (pS400) . The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
WB Description
Western blot analysis of Progesterone Receptor (pS400) expression in mouse testis (A), rat ovary (B), A2780 (C), Hela (D) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of Progesterone Receptor (pS400) staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
5241
Gene ID (Rat)
25154
SwissProt (Human)
P06401
SwissProt (Mouse)
Q00175
SwissProt (Rat)
Q63449
Available Sizes
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