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Rabbit anti-MYOD1 (pS200) Antibody

Product Specifications

Background

Rabbit polyclonal antibody to MYOD1 (pS200)

CAS Number

9007-83-4

Synonyms

BHLHC1; MYF3; MYOD; Myoblast determination protein 1; Class C basic helix-loop-helix protein 1; bHLHc1; Myogenic factor 3; Myf-3

Host

Rabbit

Reactivity

H, M, R, B, P, S, Z

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MYOD1 (pS200) . The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IH

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

WB Description

Western blot analysis of MYOD1 (pS200) expression in EC9706 (A), mouse muscle (B) whole cell lysates.

IHC description

Immunohistochemical analysis of MYOD1 (pS200) staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

4654

Gene ID (Mouse)

17927

Gene ID (Rat)

337868

SwissProt (Human)

P15172

SwissProt (Mouse)

P10085

SwissProt (Rat)

Q02346

Available Sizes

Frequently Asked Questions

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