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Mouse anti-HIF1 beta Antibody

Product Specifications

Background

Mouse monoclonal antibody to HIF1 beta

CAS Number

9007-83-4

Synonyms

BHLHE2; Aryl hydrocarbon receptor nuclear translocator; ARNT protein; Class E basic helix-loop-helix protein 2; bHLHe2; Dioxin receptor nuclear translocator; Hypoxia-inducible factor 1-beta; HIF-1-beta; HIF1-beta

Host

Mouse

Reactivity

M

Immunogen

Recombinant protein corresponding to mouse HIF1 beta.

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of Cyclin D1 expression in C2C12 (A), rat heart (B) whole cell lysates.

IHC description

Immunohistochemical analysis of Cyclin D1 staining in mouse lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Mouse)

12443

Gene ID (Rat)

58919

SwissProt (Mouse)

P25322

SwissProt (Rat)

P39948

Available Sizes

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