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Rabbit anti-NR2F6 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to NR2F6

CAS Number

9007-83-4

Synonyms

EAR2; ERBAL2; Nuclear receptor subfamily 2 group F member 6; V-erbA-related protein 2; EAR-2

Host

Rabbit

Reactivity

H, M, R, B, D, P

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human NR2F6. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC, IF/IC, IP

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of TEL expression in CCRFCEM (A), Jurkat (B), K562 (C) whole cell lysates.

IHC description

Immunohistochemical analysis of TEL staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description

Immunofluorescent analysis of TEL staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

2120

Gene ID (Mouse)

14011

SwissProt (Human)

P41212

SwissProt (Mouse)

P97360

Available Sizes

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