Rabbit anti-Beta-catenin Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Beta-catenin
CAS Number
9007-83-4
Synonyms
CTNNB; Catenin beta-1; Beta-catenin
Host
Rabbit
Reactivity
M, R
Immunogen
Recombinant protein corresponding to mouse Beta-catenin.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/2000 - 1/5000), IH (1/200 - 1/500)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Beta-catenin expression in NIH3T3 (A), rat liver (B) whole cell lysates.
IHC description
Immunohistochemical analysis of Beta-catenin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Mouse)
12387
Gene ID (Rat)
84353
SwissProt (Mouse)
Q02248
SwissProt (Rat)
Q9WU82
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