What is the catalog number for Mouse anti-HSP70 Antibody?

The catalog number for Mouse anti-HSP70 Antibody is DL95530A.

What sizes are available for Mouse anti-HSP70 Antibody?

Mouse anti-HSP70 Antibody is available in the following size(s): 50 µL, 100 µL.

How many pack sizes does Mouse anti-HSP70 Antibody come in?

Mouse anti-HSP70 Antibody is available in 2 different pack sizes.

Is Mouse anti-HSP70 Antibody in stock?

Mouse anti-HSP70 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Mouse anti-HSP70 Antibody

Product Specifications

Background

Mouse monoclonal antibody to HSP70

CAS Number

9007-83-4

Synonyms

HSPA1L; Heat shock 70 kDa protein 1-like; Heat shock 70 kDa protein 1L; Heat shock 70 kDa protein 1-Hom; HSP70-Hom; HSPA1A; HSPA1; HSPA1B; Heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1/2; HSP70-1/HSP70-2; HSP70.1/HSP70.2

Host

Mouse

Reactivity

H, M, R

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence of human HSP70. The exact sequence is proprietary.

Clonality

Monoclonal

Applications

WB, IHC, IF/IC

Concentration

1mg/mL

Dilution

WB (1/1000 - 1/2000), IH (1/100 - 1/200), IF/IC (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of HSP70 expression in Hela (A), mouse brain (B) whole cell lysates.

IHC description

Immunohistochemical analysis of HSP70 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description

Immunofluorescent analysis of HSP70 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue) .