Mouse anti-HSP70 Antibody
Product Specifications
Background
Mouse monoclonal antibody to HSP70
CAS Number
9007-83-4
Synonyms
HSPA1L; Heat shock 70 kDa protein 1-like; Heat shock 70 kDa protein 1L; Heat shock 70 kDa protein 1-Hom; HSP70-Hom; HSPA1A; HSPA1; HSPA1B; Heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1/2; HSP70-1/HSP70-2; HSP70.1/HSP70.2
Host
Mouse
Reactivity
H, M, R
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence of human HSP70. The exact sequence is proprietary.
Clonality
Monoclonal
Applications
WB, IHC, IF/IC
Concentration
1mg/mL
Dilution
WB (1/1000 - 1/2000), IH (1/100 - 1/200), IF/IC (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of HSP70 expression in Hela (A), mouse brain (B) whole cell lysates.
IHC description
Immunohistochemical analysis of HSP70 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description
Immunofluorescent analysis of HSP70 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue) .
Gene ID (Human)
3305; 3303; 3304
Gene ID (Mouse)
15482; 193740
Gene ID (Rat)
24963; 24472
SwissProt (Human)
P34931; P08107
SwissProt (Mouse)
P16627; Q61696
SwissProt (Rat)
P55063; Q07439
Available Sizes
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
