Mouse anti-Beta-tubulin Antibody
Product Specifications
Background
Mouse monoclonal antibody to Beta-tubulin
CAS Number
9007-83-4
Synonyms
TUBB4; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-III
Host
Mouse
Reactivity
H, M, R, Mk, D, C, Rb, S, Insect, Yeast
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence of human Beta-tubulin. The exact sequence is proprietary.
Clonality
Monoclonal
Applications
WB, IHC, IF/IC
Concentration
1mg/mL
Dilution
WB (1/2000 - 1/5000), IH (1/100 - 1/200), IF/IC (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Beta-tubulin expression in A549 (A), mouse brain (B), rat brain (C), chicken lung (D), rabbit testis (E), sheep muscle (F) whole cell lysates.
IHC description
Immunohistochemical analysis of Beta-tubulin staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description
Immunofluorescent analysis of Beta-tubulin staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue) .
Gene ID (Human)
10381
Gene ID (Mouse)
22152
Gene ID (Rat)
246118
SwissProt (Human)
Q13509
SwissProt (Mouse)
Q9ERD7
SwissProt (Rat)
Q4QRB4
Available Sizes
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