Rabbit anti-Kir3.1 (pS185) Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Kir3.1 (pS185)
CAS Number
9007-83-4
Synonyms
GIRK1; G protein-activated inward rectifier potassium channel 1; GIRK-1; Inward rectifier K (+) channel Kir3.1; Potassium channel, inwardly rectifying subfamily J member 3
Host
Rabbit
Reactivity
H, M, R, B, C, Mk
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Kir3.1. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Kir3.1 (pS185) expression in A549 (A), Raw264.7 (B), COS7 (C) whole cell lysates.
IHC description
Immunohistochemical analysis of Kir3.1 (pS185) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description
Immunofluorescent analysis of Kir3.1 (pS185) staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue) .
Gene ID (Human)
3760
Gene ID (Mouse)
16519
Gene ID (Rat)
50599
SwissProt (Human)
P48549
SwissProt (Mouse)
P63250
SwissProt (Rat)
P63251
Available Sizes
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