What is the catalog number for Rabbit anti-SIAH1/2 Antibody?

The catalog number for Rabbit anti-SIAH1/2 Antibody is DL92491A.

What sizes are available for Rabbit anti-SIAH1/2 Antibody?

Rabbit anti-SIAH1/2 Antibody is available in the following size(s): 50 µL, 100 µL.

How many pack sizes does Rabbit anti-SIAH1/2 Antibody come in?

Rabbit anti-SIAH1/2 Antibody is available in 2 different pack sizes.

Is Rabbit anti-SIAH1/2 Antibody in stock?

Rabbit anti-SIAH1/2 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Rabbit anti-SIAH1/2 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to SIAH1/2

CAS Number

9007-83-4

Synonyms

SIAH1; HUMSIAH; E3 ubiquitin-protein ligase SIAH1; Seven in absentia homolog 1; Siah-1; Siah-1a; SIAH2; E3 ubiquitin-protein ligase SIAH2; Seven in absentia homolog 2; Siah-2; hSiah2

Host

Rabbit

Reactivity

H, M, R, C, Z

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SIAH1/2. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of SIAH1/2 expression in HeLa (A), Jurkat (B) whole cell lysates.

IHC description

Immunohistochemical analysis of SIAH1/2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description

Immunofluorescent analysis of SIAH1/2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.