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Rabbit anti-ACTN1/2/3/4 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to ACTN1/2/3/4

CAS Number

9007-83-4

Synonyms

ACTN1; Alpha-actinin-1; Alpha-actinin cytoskeletal isoform; F-actin cross-linking protein; Non-muscle alpha-actinin-1; ACTN2; Alpha-actinin-2; Alpha-actinin skeletal muscle isoform 2; F-actin cross-linking protein; ACTN3; Alpha-actinin-3; Alpha-actinin skeletal muscle isoform 3; F-actin cross-linking protein; ACTN4; Alpha-actinin-4; F-actin cross-linking protein; Non-muscle alpha-actinin 4

Host

Rabbit

Reactivity

H, M, R, B, C, Z

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human ACTN1/2/3/4. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC, IP

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of ACTN1/2/3/4 expression in HeLa (A), HuvEc (B), mouse muscle (C), PC12 (D) whole cell lysates.

IHC description

Immunohistochemical analysis of ACTN1/2/3/4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

87; 88; 89; 81

Gene ID (Mouse)

109711; 11472; 11474; 60595

Gene ID (Rat)

81634; 63836

SwissProt (Human)

P12814; P35609; Q08043; O43707

SwissProt (Mouse)

Q7TPR4; Q9JI91; O88990; P57780

SwissProt (Rat)

Q9Z1P2; Q9QXQ0

Available Sizes

Frequently Asked Questions

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