Rabbit anti-Lamin A/C Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Lamin A/C
CAS Number
9007-83-4
Synonyms
LMN1; Prelamin-A/C
Host
Rabbit
Reactivity
H, M, R, C, P
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Lamin A/C. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Lamin A/C expression in HeLa colchicine-treated (A), mouse liver (B), rat kidney (C) whole cell lysates.
IHC description
Immunohistochemical analysis of Lamin A/C staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description
Immunofluorescent analysis of Lamin A/C staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
4000
Gene ID (Mouse)
16905
Gene ID (Rat)
60374
SwissProt (Human)
P02545
SwissProt (Mouse)
P48678
SwissProt (Rat)
P48679
Available Sizes
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
E-UNEL-M0063-01 5x 96 Tests
Uncoated Mouse GzmB (Granzyme B) ELISA Kit
Sign In for Pricing
View Details E-UNEL-M0063-02 15x 96 Tests
Uncoated Mouse GzmB (Granzyme B) ELISA Kit
Sign In for Pricing
View Details 
![Langerin / CD207 Recombinant Rabbit Monoclonal Antibody [PSH04-60]](https://cdn.genprice.uk/products/992/68dd1c8c061270361ea81b61/imageurl/ha722159.webp)
HA722159 100 µL
Langerin / CD207 Recombinant Rabbit Monoclonal Antibody [PSH04-60]
Sign In for Pricing
View Details ![2-Chloro-N-(4'-chloro-5-hydroxy [1,1'-biphenyl]-2-yl)-3-pyridinecaboxamide-D4](https://cdn.genprice.uk/products/572/4280232427392/imageurl/trc-c587856-5mg.webp)
TRC-C587856-5MG 5 mg
2-Chloro-N-(4'-chloro-5-hydroxy [1,1'-biphenyl]-2-yl)-3-pyridinecaboxamide-D4
Sign In for Pricing
View Details