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Rabbit anti-GAPDH Antibody

Product Specifications

Background

Rabbit polyclonal antibody to GAPDH

CAS Number

9007-83-4

Synonyms

GAPD; Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH

Host

Rabbit

Reactivity

H, M, R, Rb, C, Mk, S, X

Immunogen

Recombinant protein corresponding to human GAPDH.

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/2000 - 1/5000), IH (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of GAPDH expression in 293T (A), NIH3T3 (B), rat brain (C), sheep muscle (D), rabbit testis (E) whole cell lysates.

IHC description

Immunohistochemical analysis of GAPDH staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

2597

Gene ID (Mouse)

100042025

Gene ID (Rat)

24383

SwissProt (Human)

P04406

SwissProt (Mouse)

P16858

SwissProt (Rat)

P04797

Available Sizes

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