Rabbit anti-HMGB1 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to HMGB1
CAS Number
9007-83-4
Synonyms
HMG1; High mobility group protein B1; High mobility group protein 1; HMG-1
Host
Rabbit
Reactivity
H, M, R, B, C, D, Mk, P
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human HMGB1. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of HMGB1 expression in HepG2 UV-treated (A) whole cell lysates.
IHC description
Immunohistochemical analysis of HMGB1 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
3146
Gene ID (Mouse)
100862258; 15289
Gene ID (Rat)
25459
SwissProt (Human)
P09429
SwissProt (Mouse)
P63158
SwissProt (Rat)
P63159
Available Sizes
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