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Rabbit anti-TRAF6 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to TRAF6

CAS Number

9007-83-4

Synonyms

RNF85; TNF receptor-associated factor 6; E3 ubiquitin-protein ligase TRAF6; Interleukin-1 signal transducer; RING finger protein 85

Host

Rabbit

Reactivity

H, M, R, B, P

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human TRAF6. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of TRAF6 expression in Hela (A), K562 (B), Rat kidney (C), Rat heart (D), mouse kidney (E) whole cell lysates.

IHC description

Immunohistochemical analysis of TRAF6 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

7189

Gene ID (Mouse)

22034

Gene ID (Rat)

311245

SwissProt (Human)

Q9Y4K3

SwissProt (Mouse)

P70196

SwissProt (Rat)

B5DF45

Available Sizes

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