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Rabbit anti-IDH3 alpha Antibody

Product Specifications

Background

Rabbit polyclonal antibody to IDH3 alpha

CAS Number

9007-83-4

Synonyms

Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial; Isocitric dehydrogenase subunit alpha; NAD (+) -specific ICDH subunit alpha

Host

Rabbit

Reactivity

H, M, R, B, Mk

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human IDH3 alpha. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IH (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of IDH3 alpha expression in MCF7 (A), HT29 (B), mouse brain (C), mouse kidney (D) whole cell lysates.

IHC description

Immunohistochemical analysis of IDH3 alpha staining in human lymph gland formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

3419

Gene ID (Mouse)

67834

Gene ID (Rat)

114096

SwissProt (Human)

P50213

SwissProt (Mouse)

Q9D6R2

SwissProt (Rat)

Q99NA5

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