Rabbit anti-Alpha-actin Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Alpha-actin
CAS Number
9007-83-4
Synonyms
ACTC; Actin, alpha cardiac muscle 1; Alpha-cardiac actin
Host
Rabbit
Reactivity
H, M, R, B, C
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Alpha-actin. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Alpha-actin expression in mouse heart (A), mouse lung (B) whole cell lysates.
IHC description
Immunohistochemical analysis of Alpha-actin staining in mouse skeletal muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
70
Gene ID (Mouse)
11464
Gene ID (Rat)
29275
SwissProt (Human)
P68032
SwissProt (Mouse)
P68033
SwissProt (Rat)
P68035
Available Sizes
Frequently Asked Questions
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