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Rabbit anti-NFYB Antibody

Product Specifications

Background

Rabbit polyclonal antibody to NFYB

CAS Number

9007-83-4

Synonyms

HAP3; Nuclear transcription factor Y subunit beta; CAAT box DNA-binding protein subunit B; Nuclear transcription factor Y subunit B; NF-YB

Host

Rabbit

Reactivity

H, M, R, B, C, D, P

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human NFYB. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of NFYB expression in A549 (A), HEK293T (B) whole cell lysates.

IHC description

Immunohistochemical analysis of NFYB staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

4801

Gene ID (Mouse)

18045

Gene ID (Rat)

25336

SwissProt (Human)

P25208

SwissProt (Mouse)

P63139

SwissProt (Rat)

P63140

Available Sizes

Frequently Asked Questions

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