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Rabbit anti-POLR3H Antibody

Product Specifications

Background

Rabbit polyclonal antibody to POLR3H

CAS Number

9007-83-4

Synonyms

KIAA1665; RPC8; DNA-directed RNA polymerase III subunit RPC8; RNA polymerase III subunit C8; DNA-directed RNA polymerase III subunit H; RNA polymerase III subunit 22.9 kDa subunit; RPC22.9

Host

Rabbit

Reactivity

H, M, R, B, Mk

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human POLR3H. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of POLR3H expression in HEK293T (A), mouse heart (B) whole cell lysates.

IHC description

Immunohistochemical analysis of POLR3H staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

171568

Gene ID (Mouse)

78929

SwissProt (Human)

Q9Y535

SwissProt (Mouse)

Q9D2C6

Available Sizes

Frequently Asked Questions

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