Rabbit anti-c-Met Antibody
Product Specifications
Background
Rabbit polyclonal antibody to c-Met
CAS Number
9007-83-4
Synonyms
Hepatocyte growth factor receptor; HGF receptor; HGF/SF receptor; Proto-oncogene c-Met; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met
Host
Rabbit
Reactivity
H, M, R, Rb, S
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human c-Met. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of c-Met expression in HeLa (A), A431 (B) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of c-Met staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue) .
Gene ID (Human)
4233
Gene ID (Rat)
24553
SwissProt (Human)
P08581
SwissProt (Mouse)
P16056
SwissProt (Rat)
P97523
Available Sizes
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
VAng-Lsx5537-500gEcoli 500 µg (E. coli)
Recombinant Brucella Ovis rpmB Protein (aa 1-97)
Sign In for Pricing
View Details 
