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Rabbit anti-Cofilin Antibody

Product Specifications

Background

Rabbit polyclonal antibody to Cofilin

CAS Number

9007-83-4

Synonyms

CFL1; CFL; Cofilin-1; 18 kDa phosphoprotein; p18; Cofilin, non-muscle isoform; CFL2; Cofilin-2; Cofilin, muscle isoform

Host

Rabbit

Reactivity

H, M, R, P, S, Z

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Cofilin. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of Cofilin expression in K562 (A), SHSY5Y (B), rat muscle (C) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of Cofilin staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

1073; 1072

Gene ID (Mouse)

12631; 12632

Gene ID (Rat)

29271

SwissProt (Human)

P23528; Q9Y281

SwissProt (Mouse)

P18760; P45591

SwissProt (Rat)

P45592

Available Sizes

Frequently Asked Questions

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