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Rabbit anti-PDK2 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to PDK2

CAS Number

9007-83-4

Synonyms

PDHK2; [Pyruvate dehydrogenase (acetyl-transferring) ] kinase isozyme 2, mitochondrial; Pyruvate dehydrogenase kinase isoform 2; PDH kinase 2; PDKII

Host

Rabbit

Reactivity

H, M, R, B, Mk

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PDK2. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of PDK2 expression in SHSY5Y (A), rat kidney (B) whole cell lysates.

IHC description

Immunohistochemical analysis of PDK2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

5164

Gene ID (Mouse)

18604

Gene ID (Rat)

81530

SwissProt (Human)

Q15119

SwissProt (Mouse)

Q9JK42

SwissProt (Rat)

Q64536

Available Sizes

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