Rabbit anti-SMAD1/9 (pS465/467) Antibody
Product Specifications
Background
Rabbit polyclonal antibody to SMAD1/9 (pS465/467)
CAS Number
9007-83-4
Synonyms
SMAD1; BSP1; MADH1; MADR1; Mothers against decapentaplegic homolog 1; MAD homolog 1; Mothers against DPP homolog 1; JV4-1; Mad-related protein 1; SMAD family member 1; SMAD 1; Smad1; hSMAD1; Transforming growth factor-beta-signaling protein 1; BSP-1
Host
Rabbit
Reactivity
H, M, R, B, Z
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human SMAD1/9 (pS465/467) . The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of SMAD1/9 (pS465/467) expression in HeLa (A) whole cell lysates.
IHC description
Immunohistochemical analysis of SMAD1/9 (pS465/467) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
4086
Gene ID (Mouse)
17125
SwissProt (Human)
Q15797; O15198
SwissProt (Mouse)
P70340
SwissProt (Rat)
P97588
Available Sizes
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