Rabbit anti-Nogo Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Nogo
CAS Number
9007-83-4
Synonyms
KIAA0886; NOGO; Reticulon-4; Foocen; Neurite outgrowth inhibitor; Nogo protein; Neuroendocrine-specific protein; NSP; Neuroendocrine-specific protein C homolog; RTN-x; Reticulon-5
Host
Rabbit
Reactivity
H, M, R, B, D, Mk, Rb, S
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Nogo. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Nogo expression in HEK293T (A) whole cell lysates.
IHC description
Immunohistochemical analysis of Nogo staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
57142
Gene ID (Mouse)
68585
Gene ID (Rat)
83765
SwissProt (Human)
Q9NQC3
SwissProt (Mouse)
Q99P72
SwissProt (Rat)
Q9JK11
Available Sizes
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