Rabbit anti-POLR2E Antibody
Product Specifications
Background
Rabbit polyclonal antibody to POLR2E
CAS Number
9007-83-4
Synonyms
DNA-directed RNA polymerases I, II, and III subunit RPABC1; RNA polymerases I, II, and III subunit ABC1; DNA-directed RNA polymerase II 23 kDa polypeptide; DNA-directed RNA polymerase II subunit E; RPB5 homolog; XAP4
Host
Rabbit
Reactivity
H, M, R, B, Mk
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human POLR2E. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of POLR2E expression in A549 (A), Jurkat (B), HeLa (C), PC12 (D) whole cell lysates.
IHC description
Immunohistochemical analysis of POLR2E staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
5434
Gene ID (Mouse)
66420
Gene ID (Rat)
690966
SwissProt (Human)
P19388
SwissProt (Mouse)
Q80UW8
SwissProt (Rat)
B0BNE2
Available Sizes
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
