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Rabbit anti-NFIA Antibody

Product Specifications

Background

Rabbit polyclonal antibody to NFIA

CAS Number

9007-83-4

Synonyms

NFIA; KIAA1439; Nuclear factor 1 A-type; NF1-A; Nuclear factor 1/A; CCAAT-box-binding transcription factor; CTF; Nuclear factor I/A; NF-I/A; NFI-A; TGGCA-binding protein; NFIB; Nuclear factor 1 B-type; NF1-B; Nuclear factor 1/B; CCAAT-box-binding transcription factor; CTF; Nuclear factor I/B; NF-I/B; NFI-B; TGGCA-binding protein; NFIC; NFI; Nuclear factor 1 C-type; NF1-C; Nuclear factor 1/C; CCAAT-box-binding transcription factor; CTF; Nuclear factor I/C; NF-I/C; NFI-C; TGGCA-binding protein; NFIX; Nuclear factor 1 X-type; NF1-X; Nuclear factor 1/X; CCAAT-box-binding transcription factor; CTF; Nuclear factor I/X; NF-I/X; NFI-X; TGGCA-binding protein

Host

Rabbit

Reactivity

H, M, R, B, C, P, Z

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human NFIA. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of NFIA expression in Jurkat (A), Raw264.7 (B), rat brain (C) whole cell lysates.

IHC description

Immunohistochemical analysis of NFIA staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

4774; 4781; 4782; 4784

Gene ID (Mouse)

18027; 18028; 18029; 18032

Gene ID (Rat)

25492

SwissProt (Human)

Q12857; O00712; P08651; Q14938

SwissProt (Mouse)

Q02780; P97863; P70255; P70257

SwissProt (Rat)

P09414

Available Sizes

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