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Rabbit anti-Fibronectin Antibody

Product Specifications

Background

Rabbit polyclonal antibody to Fibronectin

CAS Number

9007-83-4

Synonyms

FN; Fibronectin; FN; Cold-insoluble globulin; CIG

Host

Rabbit

Reactivity

H, M, R, B, D, Rb

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Fibronectin. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of Fibronectin expression in HeLa (A), SP2/0 (B), rat lung (C) whole cell lysates.

IHC description

Immunohistochemical analysis of Fibronectin staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

2335

Gene ID (Mouse)

14268

Gene ID (Rat)

14268

SwissProt (Human)

P02751

SwissProt (Mouse)

P11276

SwissProt (Rat)

P04937

Available Sizes

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