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Rabbit anti-ATF3 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to ATF3

CAS Number

9007-83-4

Synonyms

Cyclic AMP-dependent transcription factor ATF-3; cAMP-dependent transcription factor ATF-3; Activating transcription factor 3

Host

Rabbit

Reactivity

H, M, R, B, C, D, P

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human ATF3. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of ATF3 expression in HeLa (A), mouse heart (B), rat heart (C) whole cell lysates.

IHC description

Immunohistochemical analysis of ATF3 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

467

Gene ID (Mouse)

11910

Gene ID (Rat)

25389

SwissProt (Human)

P18847

SwissProt (Mouse)

Q60765

SwissProt (Rat)

P29596

Available Sizes

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